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KMID : 1144820220280030195
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대한의생명과학회지
2022 Volume.28 No. 3 p.195 ~ p.199
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Evaluation of an Appropriate Replacement Cycle for Copper Antibacterial Film to Prevent Secondary Infection
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Je Min-A
Park Hee-Chul
Kim Jun-Seong
Lee Eun-Ju
Jung Min-Ju
Kim Min-Ji
Jeong Min-Gyoung
Yun Ji-Yun
Sin Ha-Yeon
Jin Hyun-Woo
Lee Kyung-Eun
Kim Jung-Ho
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Abstract
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The use of copper antibacterial films as an effective infection prevention method is increasing owing to its ability to reduce the risk of pathogen transmission. In this study, we evaluated the bacterial contamination of the antibacterial copper membrane attached to a door handle at a university over time. Six mounting locations with high floating population were selected. In three sites, the door handles with the antibacterial film were exposed, while the remaining three were not attached with the antibacterial films. On days 7 and 14, isolated bacterial strains were inoculated in BHI broth and agar, respectively. Colony-forming units (CFU) were determined after incubation. Strain identification was performed using bacterial 16s rRNA PCR and sequencing. Results showed that the bacterial population on day 14 significantly increased from 6 × 109 CFU/mL (day 7) to 2 × 1010 CFU/mL. Furthermore, strain distribution was not different between the on and off the copper antibacterial film groups. In conclusion, although copper has an antibacterial activity, microbial contamination may occur with prolonged use.
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KEYWORD
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Antibacterial copper film, Contamination, Bacterial 16S rRNA
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